In vitro analysis regarding the safety of components used in a film-based therapeutic system loaded with meglumine antimoniate and its activity toward Leishmania major experimental infections

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2013 vol. 35 p. 321 - 321

ue to its biocompatibility properties and its ability to promote the scar healing process, chitosan is employed in tissue engineering for the manufacture of formulations. To control the characteristic skin ulcers of cutaneous leishmaniasis, the use of a biopolymeric system that favors the scar healing process and releases an active agent such as meglumine antimoniate may be a better option. For these reasons, in this study, the cytotoxic capabilities of medium molecular weight chitosan (MMWC), lactic acid (LA) and polyvinylpyrrolidone (PVP) were evaluated as excipients in the formulation of a film-based therapeutic system that releases meglumine antimoniate. The system was tested using human macrophages (MDMs), L929 fibroblasts and Leishmania major promastigotes and intracellular amastigotes. The efficiency of this system and its ability to modulate the production of cytokines that are involved in the scar healing process were compared with conventional formulations. MMWC was